DYNAMIQUE DE LA FERMENTATION DES GRAINES DE BISSAP POUR LA PRODUCTION DU YANYANKU ET DU IKPIRU, DEUX FERMENTS TRADITIONNELS PRODUITS AU BÉNIN
P. B. AGBOBATINKPO*, P. AZOKPOTA*, J. D. HOUNHOUIGAN*
DOI:
https://doi.org/10.2023/fpjf5t07Keywords:
Yanyanku, Ikpiru, Fermentation solide, Microorganismes, Durée optimaleAbstract
Le Yanyanku et l’Ikpiru, deux ferments traditionnels utilisés respectivement pour la production des
condiments Sonru et Iru, sont des produits de la fermentation spontanée des graines de bissap (Hibiscus
sabdariffa). Cette étude vise à identifier la durée optimale de fermentation et les principaux
microorganismes impliqués dans la fermentation des graines de bissap à l’échelle traditionnelle. La charge
microbienne a été déterminée par les méthodes classiques de dénombrement au cours de la fermentation
en milieu solide. Les Bacillus spp. et les bactéries lactiques (BL) sont les principaux microorganismes
impliqués dans la fermentation, avec une charge significativement prédominante des Bacillus spp. (p <
0,01). La charge en Bacillus spp. et en BL après 96 h de fermentation était respectivement de 9,7 Log10
ufc/g et 3,8 Log10 ufc/g pour le Yanyanku et de 9,7 Log10 ufc/g et 4,5 Log10 ufc/g pour l’Ikpiru. Le pH avait
une tendance à la hausse avec une valeur moyenne de 8,4 à 96 heures, quel que soit l’additif. Aucune
différence significative n’a été notée entre les ferments prélevés de 36 à 96 h de fermentation suggérant
ainsi une durée optimale de 36 h de fermentation. La charge en Bacillus spp. et en BL à 36 h de
fermentation était respectivement de 9,1 et 3,9 Log10 ufc/g pour le Yanyanku et de 9,6 et 3,8 Log10 ufc/g
pour l’Ikpiru. Des études ultérieures sont nécessaires pour identifier au cours de la fermentation les
espèces des principaux genres dénombrés dans cette étude.
References
ADAMU, SHAHIDAH A., FAROUQ A. A., MAGASHI M. A., SOKOTO A. M. & SALAU, I. A. 2019.
Isolation and Identification of Microorganisms Encountered in Traditional Fermentation of
Soya Beans and Roselle seeds for the production of Food Tasty condiment (Dawadawa).
Greener Journal of Microbiology and Antimicrobials Vol. 4(1) : 1-6
AGBOBATINKPO B. P., AZOKPOTA P., AKISSOE N., KAYODÉ P., DA GBADJI R. & HOUNHOUIGAN
J. D. 2011. Indigenous perception and characterization of yanyanku and ikpiru two functional
additives for the fermentation of African locust bean. Ecol. Food. Nutr, 50: 101-114
AGBOBATINKPO P. B., DABADÉ S. D., LALEYÈ F., AKISSOE N., AZOKPOTA P. & HOUNHOUIGAN J.
D. 2012. Softening effect of Ikpiru and Yanyanku, two traditional additives used for the
fermentation of African Locust Bean (Parkia biglobosa) seeds in Benin Int. J. Biol. Chem. Sci.,
: 1281-1292.
ANNOUS B. A., KOZEMPEL M. F & KURANTZ M. J. 1999. Changes in membrane fatty acid composition
of Pediococcus sp strain NRRL B-2354 in reponse growth conditions and its effect on thermal
resistance. Appl Environ Microbiol 65: 2857-2862.
AZOKPOTA P., HOUNDENOUKON M. S. E., HOUNHOUIGAN J. D., NAGO C. M. & JAKOBSEN M.
Evaluation of Yanyanku processing, an additive used as starter culture to produce
fermented food condiments in Benin. J. of Food Proc. and Preserv, 35 (6): 813-821.
AZOKPOTA P., MOLLER P., HOUNHOUIGAN D. J. & JAKOBSEN, M. 2007. Biodiversity of predominant
Bacillus isolated from Afitin, Iru and Sonru at different fermentation time. Int. J. Biol. Chem.
Sci, 1: 211-222.
Dynamique de la fermentation des graines de bissap
AZOKPOTA P., HOUNHOUIGAN D. J. & NAGO M. C. 2006a. Microbiological and chemical changes
during the fermentation of African locust bean Parkia biglobosa, to produce afitin, iru and
sonru, three traditional condiments produced in Benin, Int J Food Microbiol 107: 304–309.
CHADARE F. J., GAYET D. P., AZOKPOTA P., NOUT M. J. R., LINNEMANN A. R., HOUNHOUIGAN J.
D., VAN BOEKEL, M. A. J. S. 2010. Three traditional fermented baobab foods from Benin.
Mutchayan. Dikouanyouri and Tayohounta: Preparation properties and consumption. Ecol.
Food. Nutr, 49: 1-19.
CHELULE P. K., MOKOENA M. P. & GQALENI N. 2010. Advantages of traditional lactic acid bacteria
fermentation of food in Africa. Current Research, Technology and Education Topics in Applied
Microbiology and Microbial Biotechnology A. Méndez-Vilas (Ed.), 1160 – 1167.
DAKWA S., SAKYI-DAWSON E., DIAKO C., ANNAN N.T. & AMOA-AWUA W. K. 2005. Effect of boiling
and roasting on the fermentation of soybeans into dawadawa soy-dawadawa. Inter J Food
Microbiol 104: 69–82.
EL SHEIKHA A. F & MONTET D. 2014. African Fermented Food: Historical Roots and Real Benefits: 248
In: Ray R. C & Montet D Microorganisms and Fermentation of Traditional Foods. Food
Biology series, CRC Press Taylor & Francis Group, Boca Raton, USA.
HOUBEN J. H. & TJEERDSMAVAN BOKHOVEN J. L. M. 2004. Growth inhibition of heat-injured
Enterococcus faecium by oligophosphates in a cured meat model. International Journal of Food
Microbiology, 97: 85-91
HOUNHOUIGAN D. J., NOUT M. J. R., NAGO C. M. HOUBEN J. H. & ROMBOUTS F. M. 1993.
Characterization and frequency distribution of species of lactic acid bacteria involved in the
processing of mawè, a fermented maize dough from Benin. International Journal of Food
Microbiology, 18 (3): 279 – 287.
IBRAHIM A. D., SANI A., ALIERO A. A. & SHINKAFI SA. 2011. Biocatalysis of H. sabdariffa during
dawadawan botso production and biogenaration of volatile compounds. International journal of
Biology and Chemical Science. 5(5): 1922-1931
MAHGOUB S. O. & ELBASHIR H. Z. E. 2009. Proximate composition of Karkadeh (Hibiscus sabdariffa)
seeds and some functional properties of seed protein isolate as influenced by pH and NaCl.
International Journal of Food Sciences and Nutrition, 60 (3): 183-194.
MOHAMADOU B. A., MBOFUNG C. M. F & THOUVENOT D. 2007. Functional potential of a product
from traditional biotechnology antioxidant and probiotic potential of Mbuja, produced by
fermentation of Hibiscus sabdariffa seeds in Cameroon. Journal of Food Technology, 5: 164-168.
N DIR B., GNINGUE R. D., KEITA N. D. G., SOUANE M., LAURENT L., CORNELIUS C. & THONART
P. 1997. Caractéristiques microbiologiques et organoleptiques du nététu du commerce. Cahier
Agriculture, 6: 229–304.
NOUT M. J. R., ROMBOUTS F. M. & HAVELAAR. A. 1989. Effect of accelerated natural lactic
fermentation of infant food ingredients on some pathogenic microorganisms. International
Journal of Food Microbiology 8: 355-361.
ODUNFA S. A. & ADEWUYI E. Y. 1985. Optimization of Process conditions for the fermentation of African
locust bean (Parkia biglobosa). Effect of time, temperature and humidity. Mikrobiologie
Technologie Lebensm, 9: 6-10.
OMAFUVBE B. O., FALADE O. S., OSUNTOGUN B. A. & ADEWUSI S. R. A. 2004. Chemical and
biochemical changes in African locus bean (Parkia biglobosa) and Melon (Citrus vulgaris) seeds
during fermentation to condiments. Pakistan Journal of Nutrition 3: 140-145.
OUOBA L. I. I., NYANGA-KOUMOU C. A. G., PARKOUDA C., SAWADOGO H., KOBAWILA S.C. et al.
Genotypic diversity of lactic acid bacteria isolated from African traditional alkaline
fermented foods. Journal of Applied Microbiology 108: 2010-2029.
OUOBA I. I. L., LEI V., JENSEN B. L. 2008a. Resistance of potential probiotic lactic acid bacteria and
bifidobacteria of African and European origin to antimicrobials: Determination and
transferability of the resistance genes to other bacteria. International Journal of Food
Microbiology, 121: 217-224.
Agbobatinkpo et al.
OUOBA L. I. I., DIAWARA B., JESPERSEN L. & JAKOBSEN M. 2007. Antimicrobial activity of Bacillus
subtilis and Bacillus pumilus during the fermentation of African locust bean Parkia biglobosa,
for soumbala production, J Appl Microbiol 102: 963–970.
OUOBA L. I. I., CANTOR M. D., DIAWARA B., TRAORE A. S. & JAKOBSEN M. 2003b Degradation of
African locust bean oil by Bacillus subtilis and Bacillus pumilus isolated from Soumbala a
fermented African locust bean condiment. J Appl Microbiol, 95: 868-873
YAGOUB A. A. & MOHAMMED A. M. 2008. Furundu, a meat substitute from fermented roselle
(Hibiscuss sabdariffa L) seed: Investigation on amino acids composition, protein fractions,
minerals content and HCl-extractability and microbial growth. Pakistan Journal of Nutrition,
: 352-358.
YAGOUB A. A., MOHAMED E. B., AHMED A. H. R., & EL TINAY A. E. 2004. Study on furundu, a
traditional Sudanese fermented roselle (Hibiscus sabdariffa L.) seed: effect on in vitro protein
digestibility, chemical composition, and functional properties of the total proteins. J. Agric.
Food Chem, 52: 6143–6150.
